Reporter
mTQ

Part:BBa_K2722003

Designed by: Enikö Baligács   Group: iGEM18_Munich   (2018-10-09)


mTurquoise - monomeric turquoise fluorescent protein

The protein mTurquoise is a bright cyan fluorescent protein.

The protein molecular weight is 26.88 kilodaltons.

Excitation wavelength: 434 nm

Emission wavelength: 474 nm

Extinction coefficient: 30,000 1/Mcm

Quantum yield: 0.84

Brightness: 25.2

pKa value: 4,5

fluorescence lifetime: 3.7

This protein is very well suited for a fluorescence resonance energy transfer (FRET) donor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part
//awards/part_collection
//cds
//cds/reporter
//cds/reporter/cfp
//classic/measurement/uncategorized
//function/reporter/fluorescence
Parameters
colorTurquoise
emission474 nm
excitation434 nm
familyCyan Fluorescent Proteins - CFP